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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important purpose in marketing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two supplemental splice variants have been reported but in-depth nucleotide sequence data isn't still obtainable.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide usually includes the epitope regarded from the antibody.
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This guideline outlines further more achievable optimization of mobile and immunoassay parameters to be certain the absolute best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs to your really conserved cyclin family members, whose customers are characterised by a spectacular periodicity in protein abundance in the mobile cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinctive expression and degradation patterns which contribute for the temporal coordination of each mitotic occasion. Cyclin E sorts a complex with and capabilities for a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates for the G1-S phase boundary and is degraded as cells progress via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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